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تأثیر عنصر کادمیوم بر برخی از فاکتورهای استرس اکسیداتیو در گیاه Brassica oleracea cv. saccata درمحیط هیدروپونیک | ||
| زیست شناسی کاربردی | ||
| مقاله 4، دوره 31، شماره 1 - شماره پیاپی 55، بهار 1397، صفحه 57-74 اصل مقاله (1339 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2018.3676 | ||
| نویسندگان | ||
| معصومه برجیان1؛ مریم خوش سخن مظفر2؛ معصومه خسروی رینه3 | ||
| 1کارشناسی ارشد، واحد آشتیان، دانشگاه آزاد اسلامی ، آشتیان، ایران | ||
| 2استادیار گروه زیست شناسی، واحد قم ، دانشگاه آزاد اسلامی ، قم، ایران | ||
| 3استادیار گروه زیست شناسی، واحد آشتیان ، دانشگاه آزاد اسلامی ، آشتیان، ایران | ||
| چکیده | ||
| به منظور بررسی اثر عنصر کادمیوم بر برخی از فاکتورهای استرس اکسیداتیو در گیاه Brasicca oleracea L.cv saccata در محیط هیدرپونیک آزمایشی در قالب طرح کاملاً تصادفی با سه تکرار در آزمایشگاه دانشگاه آزاد اسلامی واحد قم طراحی شد. نمونه های مورد نظر از بافت های برگ و ریشه گیاه بعد از 14 روز تیمار با کلرید کادمیوم (Ca Cl2) (5/0و 5 میکرومول در لیتر) برداشت شدند و میزان فعالیت آنزیم های سوپر اکسید دیسموتاز (SOD)، کاتالاز (CAT)، پلی فنول اکسیداز (PPO)، پراکسیداز محلول (SPO)، آسکوربات پراکسیداز (APX) و همچنین شاخص پراکسیداسیون لیپیدهای غشایی در اندام هوایی و ریشه و میزان فعالیت آنتوسیانین و کلروفیل a و b در اندام هوایی مورد ارزیابی قرار گرفت. نتایج نشان داد که میزان فعالیت آنزیم های سوپراکسید دیسموتاز در اندام هوایی در غلظت های 5/0 و 5 کادمیوم و کاتالاز در اندام هوایی و ریشه در غلظت 5 کادمیوم در مقایسه با گیاهان گروه کنترل افزایش معنی داری نشان داد. پلی فنل اکسیداز افزایش معنی داری در غلظت 5 کادمیوم و همچنین کاهش معنی داری در غلظت 5/0 کادمیوم در اندام هوایی در مقایسه با گروه شاهد نشان داد. همچنین میزان پراکسیداسیون لیپیدهای غشایی در اندام هوایی در غلظت 5/0 کادمیوم و در ریشه در غلظت 5 کادمیوم نسبت به گروه شاهد کاهش معنی داری یافت. میزان آنتوسیانین کل در اندام هوایی در گیاهان تحت تیمار با غلظت های 5/0و 5 کادمیوم نسبت به گروه شاهد کاهش معنی داری نشان داد و بررسی میزان کلروفیل a و b برگ ها نشان داد که میزان تجمع در اندام هوایی با افزایش معنی دار برای غلظت 5 کادمیوم همراه است. بنابراین نتایج به دست آمده نشان داد که کادمیوم در شرایط تنش اکسیداتیو بر روی برخی از فاکتورهای سیستم آنتی اکسیدان گیاه اثر داشته است. | ||
| کلیدواژه‌ها | ||
| سمیت کادمیوم؛ Brassica oleracea؛ تنش اکسیداتیو؛ محیط هیدرو پونیک | ||
| عنوان مقاله [English] | ||
| Effect of Cadmium on some oxidative stress factors in Brassica oleracea l. cv. saccata in hydroponic culture | ||
| نویسندگان [English] | ||
| Masoumeh Borjian1؛ Maryam Khoshsokhan Mozaffar2؛ Masoumeh khosravireyneh3 | ||
| 1Department of Biology, Ashtian branch ,Islamic Azad University , Ashtian, Iran | ||
| 2Department of Biology, Qom branch, Islamic Azad University ,Qom, Iran | ||
| 3Department of Biology, Ashtian branch, Islamic Azad University , Ashtian, Iran | ||
| چکیده [English] | ||
| Purpose of this study is determine the effect of cadmium on some oxidative stress factors in Brassica oleracea l. cv. saccata in a random pattern & repeated 3 times. in order to control experimental conditions the same as the inhibitory effects of the other factors such as soil nutrition factors the hydroponic growth culture was used. The root and shoot samples picked up in 14th day after treatment with different cadmium concentration (0.5, 5) and used it for determination of biochemical and morphological parameters. The activity of Superoxide dismutase (SOD), Polyphenol Oxidase (PPO), catalase (CAT) and malondialdehyde in aerobiosis bodies and roots and also the content of Anthocyanin and chlorophyll a and b in shoots were measured. The results showed the activity of SOD in some aerobiosis bodies in 5 and 0.5 concentration of cadmium & also CAT enzyme activity in aerobiosis bodies with 5 concentration and in roots with 5 cadmium concentration has increased. The cadmium treatment have showed that PPO increased in 5 cadmium con. and also the decreased in 0.5 cadmium con., significantly, in aerobiosis bodies. The amount of Malondialdehyde as an index of proxidation membrance lipids has been decreased significantly in 0.5 con. in shoots. In caring plants with cadmium, Anthocyanin has decreased, significantly. The amount of a & b chlorophylls decreased in 5 concentration. Thus, the obtained results in this study have showed that cadmium has a positive effect on some antioxidant system parameters of the plant in the oxidative stress conditions & regarding that in the most cadmium have never seen any significant root in the data, we can conclude the cadmium conducts from the root to shoot. | ||
| کلیدواژه‌ها [English] | ||
| Cadmium toxicity, Oxidative stress, Brassica oleracea l. cv. saccata, hydroponic culture | ||
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