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اثر شوری بر آنزیمهای آنتی اکسیداتیو در ریشه‌ها و اندام هوایی گیاه ذرت (Zea mays L.) | ||
| زیست شناسی کاربردی | ||
| مقاله 1، دوره 28، شماره 1، فروردین 1394، صفحه 5-22 اصل مقاله (459.86 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2015.1964 | ||
| نویسندگان | ||
| لطیفه پوراکبر1؛ سونیا مقسومی هولاسو2 | ||
| 1استادیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه ارومیه | ||
| 2دانشجوی ارشد، گروه زیست‌شناسی، دانشکده علوم، دانشگاه ارومیه | ||
| چکیده | ||
| شوری یکی از مهمترین فاکتورهای محیطی است که موجب کاهش رشد، نمو و حاصلخیزی گیاهان در سراسر جهان میشود. موضوع تحقیق حاضر مطالعه اثر تنش شوری (0، 50 و 100 میلی مولار NaCl) بر گیاهان ذرت (Zea mays L. cv. SC. 704) کشت شده به روش گلخانه‌ای و هیدروپونیک بود. بعد از یک ماه تیمار شوری، پارامترهای رشد و برخی تغییرات بیوشیمایی در ریشه‌ها و اندام هوایی گیاهان کشت شده مورد مطالعه قرار گرفت. نتایج نشان داد که با افزایش غلظت شوری، سطح برگ وطول ریشه‌ها و اندام هوایی کاهش یافت، در صورتیکه میزان قندهای محلول، پراکسید هیدروژن، مالون دی آلدئید به عنوان شاخص پراکسیداسیون چربی افزایش یافت. میزان فعالیت آنزیمهای کاتالاز (CAT)، آسکوربات پراکسیداز (APX) و گایاکول پراکسیداز (GPX)در گیاهان تحت تیمار در هر دو غلظت کلرید سدیم افزایش یافت. بنابراین اعمال تیمار NaClبا افزایش تولید ROSموجب القا تنش اکسیداتیو و افزایش فعالیت آنزیمهای مسئول محافطت آنتی‌اکسیدانی در گیاهان ذرت شد. | ||
| کلیدواژه‌ها | ||
| ذرت؛ شوری؛ پراکسیداسیون چربی؛ تنش اکسیداتیو؛ پراکسید هیدروژن؛ آنتی اکسیدان | ||
| عنوان مقاله [English] | ||
| Salinity effect on antioxidative enzymes activity in roots and leaves of maize plant (Zea mays L. cv. SC. 704) | ||
| نویسندگان [English] | ||
| Latifeh Poorakbar1؛ sonia Maghsoumi Holasoo2 | ||
| چکیده [English] | ||
| Salinity is one of the most important environmental factors which reduce the plants growth and their fertility around the world. In this study maize (Zea mays L.) plants grown in hydroponic culture were treated with NaCl(0, 100 and 150mM).After 1 month of treatment, growth parameters and some biochemical changes were studied in roots and shoots of plants. The results showed that with increasing NaCl concentration, leaf area and root and shoot length were decreased, while total soluble sugar, level of H2O2, malondealdyde as indicator lipid of peroxidation were increased. The activities of catalase (CAT), ascorbate peroxidase (APX) and guaiacol peroxidase increased in plants supplied with both concentration of NaCl. Therefore, it can be concluded that supply of NaCl induced oxidative stress by increasing production of ROS despite increased antioxidant protection in maize plants. | ||
| کلیدواژه‌ها [English] | ||
| Maize, Salinity, Lipid peroxidation, Oxidative stress, H2O2, antioxidants | ||
| مراجع | ||
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