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حفاظت کلسیمکلرید از گیاه پریوش Catharanthus roseus تحت تنش شوری با القاء سیستم دفاع آنتیاکسیداتیو و افزایش رنگیزههای فتوسنتزی | ||
زیست شناسی کاربردی | ||
مقاله 7، دوره 30، شماره 1، شهریور 1396، صفحه 113-136 اصل مقاله (492.13 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2017.2991 | ||
نویسندگان | ||
مهری عسکری* 1؛ فریبا امینی2؛ سهیلا شکری3 | ||
1استادیار فیزیولوژی گیاهی، گروه زیست شناسی ، دانشگاه اراک، اراک ، ایران | ||
2استادیار فیزیولوژی گیاهی،گروه زیست شناسی ، دانشگاه اراک ، اراک ،ایران | ||
3دانشجوی کارشناسی ارشد ، فیزیولوژی گیاهی ، گروه زیست شناسی ، دانشگاه اراک ، اراک ، ایران | ||
چکیده | ||
این مطالعه اثرات کلسیم کلرید را به عنوان یک عامل تعدیل کننده تنش شوری بر گیاه پریوش بررسی میکند. گیاهان 49 روزه با غلظتهای مختلف کلرید سدیم (0، 35، 70 و 100 میلیمولار) به تنهایی و توام با غلظتهای مختلف کلرید کلسیم (0، 5 و 10 میلیمولار) به مدت 21 روز تیمار شدند. سپس درصد بازدارندگی رادیکال دیفنیل-2- پیکریلهیدرازیل (I%)، فعالیت آنزیمهای آنتیاکسیدان، سطح و تعداد برگ و مقدار رنگیزههای فتوسنتزی اندازهگیری شدند. نتایج کاهش معنیدار مقدار رنگیزهها، سطح و تعداد برگ را تحت تنش شوری نشان داد. افزودن کلسیم کلرید به گیاهان تحت تنش شوری، مقادیر رنگیزهها، سطح برگ، فعالیت آنزیمهای آنتیاکسیدان و I% را در مقایسه با گیاهان رشد یافته تحت تنش شوری به تنهایی افزایش داد. بیشترین افزایش در تیمارهای ترکیبی (100 میلیمولار کلرید سدیم + 5 میلیمولار کلرید کلسیم) مشاهده شد. نتایج اثرات مفید تغذیه کلسیم بر رشد گیاه پریوش تحت تنش شوری را مطرح میکند. | ||
کلیدواژهها | ||
دیفنیل--2پیکریــل هیدرازیــل؛ ســوپر اکســید دیســموتاز؛ شــاخص رشــد؛ کاتــالاز؛ گایاکولپراکســیداز | ||
عنوان مقاله [English] | ||
Protection of CaCl2 of Catharanthus roseus under salinity stress by inducing antioxidative defence system and increase of photosynthetic pigments | ||
نویسندگان [English] | ||
Mehri Assgari1؛ Fariba Amini2؛ Soheila Shokri3 | ||
1Assistant Professor of Plant Physiology, Department of Biology, Arak University, Arak, Iran | ||
2Assistant Professor of Plant Physiology, Department of Biology, Arak University, Arak, Iran | ||
3MSc Student, Plant Physiology, Department of Biology, Arak University, Arak, Iran | ||
چکیده [English] | ||
This study evaluated the effects of calcium chloride as alleviator agent in salinity stress on Catharanthus roseus. 49- day- old plants were treated with different concentrations of NaCl (0, 35, 70 and 100 mM) alone and in combination of various CaCl2 concentration (0, 5 and 10 Mm) for 21 days. Then, percent of Inhibition of 1,1-diphenyl-2- picrylhydrazyl radical (I%), activity of antioxidant enzymes, contents of photosynthetic pigments, leaf number and area were measured. The results indicated that pigments contents, leaf area and number were significantly decreased by salinity. Addition of CaCl2 to salinity-stressed plants increased of pigments contents, leaf area, the activitiy of antioxidant enzymes and I% in compared to plants grown with NaCl alone. The maximum enhancement was observed with the combined treatments (100mM NaCl + 5mM CaCl2). The results are discussed in terms of the beneficial effects of calcium supply on the growth of vinca under saline conditions. | ||
کلیدواژهها [English] | ||
Catalase, diphenyl�2�picrylhydrazyl radical (DPPH), Growth parameters, guaiacol peroxidase, superoxide dismutase | ||
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