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تولید زایلیتول توسط سویه مخمری دباریومایسس هانسنی جداسازی شده از گرده گل پنیرک | ||
| زیست شناسی کاربردی | ||
| مقاله 11، دوره 31، شماره 1 - شماره پیاپی 55، خرداد 1397، صفحه 164-177 اصل مقاله (1.1 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2017.4295. | ||
| نویسندگان | ||
| فروغ عسگری کرچگانی* 1؛ مرضیه شمعی1؛ غلامرضا قزلباش2؛ ایرج نحوی3 | ||
| 1دانشجوی کارشناسی ارشد میکروبیولوژی، دانشگاه آزاد اسلامی واحد فلاورجان. | ||
| 2استادیار میکروبیولوژی، دانشگاه شهید چمران اهواز، دانشکده علوم، گروه زیست شناسی. | ||
| 3استاد، دانشگاه اصفهان، دانشکده علوم، گروه زیست شناسی. | ||
| چکیده | ||
| زایلیتول یک پلی‌اُول پنج‌کربنه با قدرت شیرین‌کنندگی بالاست. این پلی‌اُول شیرین‌ترین پلی‌الکل می‌باشد. تولید بیوتکنولوژیکی زایلیتول با استفاده از میکروارگانیسم‌ها از نظر زیست محیطی ایمن است و به عنوان یک روش جایگزین روش شیمیایی مورد مطالعه قرار می‌گیرد. با توجه به خواص ارزشمندی که زایلیتول دارد به عنوان جایگزین قند در صنایع شیمیایی، غذایی و دارویی استفاده می‌شود. بر اساس مطالعات اخیر، در میان میکروارگانیسم‌ها، مخمرها بهترین تولیدکننده می‌باشند. در این پژوهش این شیرین‌کننده ارزشمند توسط سویه مخمری دباریومایسس هانسنی تولید شد. این مخمر از گرده گل پنیرک جداسازی و با تعیین توالی 18S rRNA و هم ردیفی با توالی های موجود در پایگاه داده ها شناسایی گردید. زایلیتول در محیط تولید حاوی زایلوز و دیگر مواد معدنی تولید شد و با روش های آنالیز کروماتوگرافی لایه نازک و با استفاده از کیت مگازایم ایرلند شناسایی و تایید و مقدار آن با روش کروماتوگرافی مایع با عملکرد بالا اندازه‌گیری گردید. سویه مخمری دباریومایسس هانسنی قادر به تولید 45/20 گرم بر لیتر زایلیتول از 40 گرم بر لیتر زایلوز، بعد از 48 ساعت بود. | ||
| کلیدواژه‌ها | ||
| زایلیتول؛ زایلوز؛ Dataryum Hansen | ||
| عنوان مقاله [English] | ||
| Production of xylitol by Debaryomyces hansenii , the yeast strain isolated from pollen of Malva sylvestris flower | ||
| نویسندگان [English] | ||
| Forough Asgary Karchegany1؛ Marzieh Shamee1؛ Gholamreza Ghezelbash2؛ Iraj Nahvi3 | ||
| 1MSc of Microbiology, Falavarjan Azad University, Isfahan, Iran. | ||
| 2Assistant Professor, Department of Biology, Faculty of Science, Shahid Chamran , Ahvaz, Iran. | ||
| 3Professor, Department of Biology, Faculty of Science, Isfahan University, Isfahan, Iran. | ||
| چکیده [English] | ||
| Xylitol is a naturally five – carbon polyol with a high sweetening power. Xylitol is the sweetest among the polyalcohols. The biotechnological method of producing xylitol by microorganisms has been studied as an alternative to the chemical method and is very environmentally safe. Xylitol is an attractive sugar substitute for use in the food-processing, nutritional supplement and pharmaceutical industries. Among the microorganisms, yeasts are considered as the best xylitol producer. The purpose of this study was xylitol production by Debaryomyces hansenii that isolated from pollen of Malva sylvestris flower. This strain was identified by 18SrRNA sequencing and following its alignment with existed similar strains in data bases. Xylitol was produced in production medium contained xylose and other compounds. Xylitol was first identified by thin layer chromatography (TLC) method then it was confirmed by Megasyme kit. Xylitol concentration was quantified using high performance liquid chromatography ( HPLC) method. This strain produced 20.45 g l-1 xylitol after 48 hours culture incubation in medium contained 40 g l-1 xylose. | ||
| کلیدواژه‌ها [English] | ||
| Debaryomyces hansenii, xylitol, xylose | ||
| مراجع | ||
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