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اثر عصاره های آبی و الکلی گیاه گزنه دوپایه بر رشد و برخی ویژگی‌های بیوشیمیایی گیاه گوجه-فرنگی در شرایط گلخانه‌ای | ||
| زیست شناسی کاربردی | ||
| دوره 35، شماره 1 - شماره پیاپی 70، خرداد 1401، صفحه 133-155 اصل مقاله (1.59 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2021.37178.1439 | ||
| نویسندگان | ||
| الهه ریاضی1؛ فرشاد رخشنده رو* 2؛ وحید عبدوسی3 | ||
| 1. دانش آموخته کارشناسی ارشد گروه باغبانی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران. | ||
| 2دانشیار گروه گیاهپزشکی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران. | ||
| 3استادیار گروه باغبانی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران. | ||
| چکیده | ||
| در این بررسی اثر عصاره های آبی و الکلی گیاه گزنه دو پایه ایران (Urtica dioica L.) بر رشد و برخی ویژگی های بیوشیمیایی گیاه گوجه فرنگی، رقم Super chef ، در شرایط گلخانه‌ای مورد بررسی قرار گرفت. برای این منظور از بافت های خشک شده ساقه و برگ گزنه عصاره های آبی و الکلی تهیه و به صورت مستقیم به ریزوسفر خاک گلدان ها تزریق و در زمان های معین پس از تیمار، برخی شاخص های بیوشیمیایی و رشدی گیاهچه های گوجه ارزیابی شد. همچنین با استفاده از آزمون RT-sq-PCR میزان بیان نسبی ژن کدکننده پذیرنده سیتوکینین SlHK4-Cytokinin inducer در نمونه ها در زمان 14روز بعد از تیمار ارزیابی گردید. نتایج نشان داد که عصاره های آبی و الکلی گزنه توانسته‌اند در غلظت ppm500 با اختلاف معنی داری موجب افزایش میزان سرعت نسبی رشد، مقدار کلروفیل های a و b، فنل کل و فعالیت اختصاصی آنزیم های پراکسیدازPOX و پلی فنل اکسیداز PPO و همچنین افزایش نسبی میزان بیان ژن SlHK4-Cytokinin inducer در مقایسه با گیاهان شاهد شوند. نتایج حاصل از این پژوهش می‌تواند به عنوان روشی کاربردی برای بهبود رشد گیاه گوجه فرنگی در شرایط گلخانه مورد استفاده قرار گیرد. | ||
| کلیدواژه‌ها | ||
| بیان ژن؛ آنزیم پراکسیداز؛ آنزیم پلی فنل اکسیداز؛ فنل؛ گیاه گزنه | ||
| عنوان مقاله [English] | ||
| The effect of the aquatic and alcoholic extracts of bipod nettle weed on the growth and some biochemical features of tomato plant under the greenhouse condition | ||
| نویسندگان [English] | ||
| Elaheh Ryazi1؛ Farshad Rakhshandehroo2؛ Vahid Abdossi3 | ||
| 1Graduated student/Department of Horticulture, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 2Associate Professor, Department of Plant Protection, Faculty of Agricultural Sciences and Food Industry, Science and Research Branch of Islamic Azad University, Tehran. | ||
| 3Assistant Professor Department of Horticulture, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad university, Tehran, Iran, | ||
| چکیده [English] | ||
| In this research the effects of bipod nettle aquatic and alcoholic extracts on the growth and some biochemical parameters of tomato plant, Super chef cultivar, were investigated under the greenhouse condition. For this purpose, aquatic and alcoholic extracts prepared from dried stems and leaves of nettle weed plant. Tomato seedlings were treated by direct injection of the extracts into the soil rhizosphere medium at the defined concentrations of 100,300 and 500 ppm. The growth and some biochemical parameters in treated tomato plants were assessed 3, 6, 9 and 14 days after treatment. The expression level of the SlHK4-Cytokinin inducer gene was also evaluated 14 days after the treatment using RT-sqPCR assay. Results showed that aquatic and alcoholic extracts of the nettle plants at the concentration of 500ppm significantly increased the relative growth rate, chlorophyll a and b concentratins, total phenol content, the specific activities of peroxidase (POX) and polyphenoloxidase (PPO) as well as the expression level of SlHK4-Cytokinin inducer gene in treated tomato plants. The results of this study can be used as an applicable method to improve the growth of the tomato plant under the greenhouse condition. | ||
| کلیدواژه‌ها [English] | ||
| Gene expression, peroxidase enzyme, polyphenol oxidase enzyme, total phenol, stinging nettle | ||
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| مراجع | ||
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