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تاثیر عصاره هیدروالکلی ریشه انجبار ( (Polygonum bistorta Lبر میزان قند خون موش های سوری دیابتی شده با استرپتوزوتوسین | ||
زیست شناسی کاربردی | ||
دوره 35، شماره 1 - شماره پیاپی 71، خرداد 1401، صفحه 156-166 اصل مقاله (466.89 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2021.37075.1437 | ||
نویسندگان | ||
الهام صالحی* 1؛ مجید مروتی شریف اباد1؛ مهدیه کریمی2؛ مهناز سازی زواره2 | ||
1استادیار،گروه علوم پایه،دانشکده پیرادامپزشکی،دانشگاه اردکان،اردکان،ایران | ||
2دانشجوی ارشد علوم جانوری گرایش سلولی- تکوینی،دانشکده پیرا دامپزشکی ، دانشگاه اردکان،اردکان ،ایران | ||
چکیده | ||
دیابت از شایع ترین بیماری های قرن حاضر است. عوارض زیاد داروهای شیمیایی مورد استفاده در درمان آن گرایش به درمان را توسط گیاهان دارویی که دارای عوارض جانبی کمتری هستند روز به روز افزایش می دهد. فلاونوییدهای موجود درگیاهان با اثر آنتی اکسیدانی خود توانایی مهار استرس اکسیداتیو و دیابت ناشی از آن را دارند. انجبار گیاهی حاوی فلاونویید است لذا در این مطالعه تاثیر عصاره هیدروالکلی ریشه انجبار بر میزان قند خون موشهای سوری دیابتی شده با استرپتوزوتوسین ارزیابی شده است. برای انجام این مطالعه، دیابت با mg/kg30 استرپتوزوتوسین به صورت داخل صفاقی در ۱۵ موش سوری القا شد و موشها به طور تصادفی به 3 گروه تقسیم شدند و یک گروه هم به عنوان کنترل در نظر گرفته شد. گروه کنترل و دیابتی، نرمالین سالین را دریافت کردند و گروه های تیمار به ترتیب mg/kg 5/0 گلی بنکلامید و mg/kg 150 عصاره هیدروالکلی ریشه انجبار را به مدت ۱۵ روز دریافت کردند. در پایان روزهای اول، هفتم و پانزدهم مطالعه، خونگیری انجام شد و میزان گلوکز خون اندازه گیری شد. داده ها با استفاده از آزمون آنالیز واریانس یک طرفه و آزمون تعقیبی توکی به کمک نرم افزار SPSS ورژن 25تحلیل شد. در این پژوهش05/0> Pدر نظر گرفته شد. داده ها حاکی از آن بود که به دنبال القای دیابت، میزان گلوکز خون در تمامی گروه های دیابتی در روزهای اول، هفتم و پانزدهم افزایش معنی داری در مقایسه با گروه کنترل داشت(05/0>P). | ||
کلیدواژهها | ||
: استروپتوزوتوسین؛ انجبار؛ دیابت شیرین؛ قندخون؛ موش سوری | ||
عنوان مقاله [English] | ||
The effect of hydroalcohlic of Bistort (Polygonum bistorta L) root on blood glucose levels of streptozotocin induced diabetic mice | ||
نویسندگان [English] | ||
elham salehi1؛ majid morovatisharifabad1؛ mahdieh karimi2؛ mahnaz sazi zavareh2 | ||
1assistant professor,Department of Basic sciences, Faculty of Veterinary Medicine, Ardakan university,Ardakan,Iran | ||
2Department of Basic sciences, Faculty of Veterinary medicine, Ardakan university, Ardakan, Iran | ||
چکیده [English] | ||
Diabetes is one of the most common disease of the present century. The many side effects of the chemical drugs used to treat it increase the tendency to be treated by herbs that have fewer side effects. Plant flavonoids with their antioxidant effect have the ability to inhibit oxidative stress and diabetes. Bistort contains flavonoids, so in this study, the effect of hydroalcoholic extract of Bistort root on blood glucose in streptozotocin diabetic mice have been assessed in the present study. In this experimental study, diabetes was induced by administration of 30 mg/kg of streptozotocin intraperitoneally in 15 mice and they were randomly divided into 3 groups. One group considered as control. The control and diabetic groups received normal saline and the treatment groups received glibenclamide at dose 0.5 mg/kg and hydroalcoholic extract of Bistort root at doses of 150 μg/kg for 15 days. At the end of the first , seventh and fifteenth days of the study, blood samples were taken and blood glucose levels were measured. The data were analysed by one way Anova and Turkey's test using SPSS 25. In this study, P<0/05 was considered.In this study, following the induction of diabetes blood glucose levels in all diabetic groups in the first, seventh and fifteenth days had a significant increase compared to control group (P<0/05). | ||
کلیدواژهها [English] | ||
Blood glucose, Diabetes mellitus, Mice, Polygonum bistorta L, Streptozotocin | ||
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مراجع | ||
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