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فعالیت زیستی عصاره جلبک های کلرلا و اسپیرولینا و زیست توده تیمار شده آن ها قبل و بعد از پروتئولیز | ||
| زیست شناسی کاربردی | ||
| مقاله 9، دوره 36، شماره 2 - شماره پیاپی 76، شهریور 1402، صفحه 141-159 اصل مقاله (1.43 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2023.43391.1559 | ||
| نویسندگان | ||
| طیبه هادی توران پشتی1؛ فخری السادات حسینی* 2؛ فاطمه صفاخواه1 | ||
| 1کارشناسی ارشد زیست فناوری میکروبی، دانشکده علوم زیستی، دانشگاه الزهراء | ||
| 2استادیار بیوتکنولوژی . دانشکده علوم زیستی . دانشگاه الزهرا (س) | ||
| چکیده | ||
| مقدمه: میکروجلبکها ارگانیسمهایی با متابولیتهای متنوع و با ارزش هستند و دو میکروجلبک اسپیرولینا و کلرلا به دلیل دارا بودن مقادیر بالای پروتئین بسیار مورد توجه قرار گرفتهاند. روش کار: در این مطالعه پس از کشت میکروجلبکهای اسپیرولینا و کلرلا، از آنها عصارهگیری شد و همچنین به وسیله سونیکاتور پروتئین آنها استخراج شده و تحت هیدرولیز آنزیمی با دو آنزیم آلکالین پروتئاز و پپسین قرار گرفتند و با استفاده از تست DPPH خاصیت آنتی اکسیدانی آنها مورد سنجش قرار گرفت. همچنین خاصیت ضد تکثیری پروتئینهای میکروجلبکهای اسپیرولینا و کلرلا قبل و بعد از هیدرولیز آنزیمی با دو لاین سلولی سرطانی MCF-7 و Caco-2 و خاصیت ضد میکروبی با دو باکتری اشریشیاکلی و استافیلوکوکوس اورئوس مورد سنجش قرار گرفت. نتیجه: میزان زنده مانی سلولهای سرطانی MCF-7پس از تیمار 48 ساعته با غلظت 200میکروگرم بر میلی لیتر پروتئین هیدرولیز شده اسپیرولینا و کلرلا به ترتیب 60/21 درصد و 65/37 درصد و این میزان برای سلول سرطانی Caco-2 به ترتیب 63/23 درصد و 48/26 درصد کاهش یافته است. همچنین نتایج نشان داد که هیدرولیز پروتئین در بهبود خواص آنتی اکسیدانی، ضد تکثیری و ضد میکروبی تأثیر مثبت داشته است. | ||
| کلیدواژهها | ||
| آنتی اکسیدانی؛ آلکالین پروتئاز؛ پپسین؛ ضد میکروبی؛ ضد تکثیر سلولی؛ ضد سرطان | ||
| عنوان مقاله [English] | ||
| Comparison of biological activity in extracts from Chlorella and Spirulina alga the related algal biomass before and after proteolysis | ||
| نویسندگان [English] | ||
| Taibeh Hadi Turan back1؛ Fakhri Sadat Hosseini2؛ fatemeh safakhah1 | ||
| 1Master's Degree in Microbial Biotechnology, Faculty of Biological Sciences, Al Zahra University | ||
| 2Assistant Professor، Department of Biotechnology, Faculty of Biological Science | ||
| چکیده [English] | ||
| Introduction: Microalgae are organisms with diverse and valuable metabolites, and two microalgae, spirulina and chlorella, have received much attention due to their high protein content. Method: In this study, after the cultivation of spirulina and chlorella microalgae, they were extracted and their protein was extracted by sonicator and subjected to enzymatic hydrolysis with two enzymes, alkaline protease and pepsin, and using the test DPPH and their antioxidant properties were measured. Also, antiproliferative property with two cancer cell lines MCF-7 and Caco-2 and antimicrobial property with two bacteria Escherichia coli and Staphylococcus aureus, proteins of spirulina and chlorella microalgae were measured before and after enzymatic hydrolysis. Result: The survival rate of MCF-7 cancer cells after 48-hour treatment with a concentration of 200 µg/ml hydrolyzed protein of S.platensis and C.vulgaris was 21.60% and 37.65%, respectively, and this amount has decreased for Caco-2 cancer cells by 23.63% and 26.48%, respectively.. Also, the results showed that protein hydrolysis had a positive effect on improving antioxidant, antiproliferative and antimicrobial properties | ||
| کلیدواژهها [English] | ||
| antioxidant, alkaline protease, pepsin, antimicrobial, anti-cell proliferation, anticancer | ||
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