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تولید استرپتوتریسین D توسط Streptomyces sp. جدا شده از ریزوسفر گیاه دارویی Mentha longifolia | ||
زیست شناسی کاربردی | ||
دوره 37، شماره 4 - شماره پیاپی 82، دی 1403 | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2023.45244.1592 | ||
نویسندگان | ||
فاطمه علی نژاد1؛ غلام خداکرمیان* 2 | ||
1دانشجوی دکتری بیماریشناسی گیاهی، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان | ||
2.استاد بیماری شناسی گیاهی، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان | ||
چکیده | ||
مقدمه: اکثر آنتیبیوتیکهایی که امروزه استفاده میشوند توسط اکتینومیستها و به ویژه جنسStreptomyces سنتز میشوند و به دلیل پتانسیل بالای آنها برای تولید ترکیبات ضدمیکروبی، این گروه از باکتریها همچنان یک منبع بالقوه برای شناسایی ترکیبات جدید هستند. به دلیل تاثیر ریزفاکتورهای محیطی بر جوامع میکروبی و بیان ژنهای آنها از جمله ژنهای سنتزکننده ترکیبات ثانویه و ترکیبات ضدمیکروبی، جداسازی Streptomyces ها از زیستگاههای منحصر به فرد با هدف شناسایی ترکیبات جدید، میتواند مفید باشد. به نظر میرسد ریزوسفر گیاهان دارویی سنتی میتواند یک محیط غنی برای جداسازی و شناسایی Streptomyces ها و ترکیبات جدید باشد. در این مطالعه به بررسی فعالیت ضدمیکروبی جدایههایStreptomyces جمعآوریشده از ریزوسفر گیاهان دارویی بومی استان همدان علیه باکتریهای گرم مثبت و گرم منفی پرداخته شد. روشها: جدایههای مختلفStreptomyces از ریزوسفر گیاهان دارویی منطقه آورزمان در استان همدان جمع آوری شدند و فعالیت ضدمیکروبی آنها علیه چند باکتری گرم مثبت و گرم منفی با استفاده از آزمونهای زیستسنجی انجام شد. آنالیز متابولومیکس عصارههای استخراج شده از کشتمایع جدایههای فعال جهت شناسایی ترکیبات ضدمیکروبی با استفاده از دستگاه ESI-MS انجام شد. نتایج و بحث: بر اساس آزمونهای زیستسنجی جدایه Streptomyces sp. 3Z جدا شده از ریزوسفر Mentha longifolia فعالیت ضدمیکروبی قوی در تقابل با تعدادی از باکتریهای گرم مثبت وگرم منفی نشان داد. آنالیز متابولومیکس فرکشن فعال بهدستآمده از عصاره استخراجی از کشت مایع این جدایه، تولید Streptothricin D توسط این جدایه را تایید کرد. | ||
کلیدواژهها | ||
Streptomyces؛ آنتی&lrm؛ بیوتیک؛ استرپتوتریسین؛ ریزوسفر؛ طیف سنجی جرمی؛ فعالیت ضد باکتریایی | ||
عنوان مقاله [English] | ||
Production of streptothricin D by a rhizospheric Streptomyces sp. isolated from the medicinal plant Mentha longifolia | ||
نویسندگان [English] | ||
fatemeh alinejad1؛ Gholam Khodakaramian2 | ||
1PhD student in Plant Pathology, Department of Phytomedicine, Faculty of Agriculture, Bu-Ali Sina University, Hamadan | ||
2Professor of Plant Pathology, Department of Phytomedicine, Faculty of Agriculture, Bu-Ali Sina University, Hamadan | ||
چکیده [English] | ||
Introduction: The majority of antibiotics that are used today are produced by actinomycetes, especially the genus of Streptomyces. Because of the high potential of Streptomyces species to produce antimicrobial compounds, these bacteria are still an interesting source for identifying new antimicrobial compounds. Due to the influence of environmental micro factors on microbial communities and their gene expression, including the synthesis of secondary and antimicrobial compounds, it is valuable to isolate Streptomyces from unique environments for identifying new compounds. The rhizosphere of wild traditional medicinal plants appears to be a promising environment for the isolation and identification of Streptomyces, as well as the discovery of new compounds... In this study, we investigated the antimicrobial activity of Streptomyces isolates collected from the rhizosphere of native medicinal plants in Hamedan province against Gram-positive and Gram-negative bacteria. Methods: Different isolates of Streptomyces were collected from the rhizosphere of medicinal plants in the Averzaman region in Hamedan province, and their antimicrobial activity against Gram-positive and Gram-negative bacteria was investigated using bioassay tests. Metabolomics analysis of the extracts obtained from the liquid culture of the active isolates was performed using ESI-MS to identify active compounds corresponding to the observed bioactivities. Results and Discussion: Based on bioassay tests, the Streptomyces sp. 3Z isolated from the rhizosphere of Mentha longifolia exhibited strong antimicrobial activity against Gram-positive and Gram-negative bacteria. Metabolomics analysis of the active fraction of the crude extract of this isolate confirmed the production of Streptothricin D by this isolate. | ||
کلیدواژهها [English] | ||
Streptomyces, Antibiotic, Streptothricin, Antibacterial activity, Rhizosphere, Mass spectrometry | ||
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